tailieunhanh - Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài:The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells. | Research Open Access The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells Helle F Jorgensen Véronique Azuara Shannon Amoils Mikhail Spivakov Anna Terry Tatyana Nesterova Bradley S Cobb Bernard Ramsahoye Matthias Merkenschlager and Amanda G Fisher Addresses Lymphocyte Development Group MRC Clinical Sciences Centre Imperial College School of Medicine London W12 0NN UK. Developmental Epigenetics MRC Clinical Sciences Centre Imperial College School of Medicine London W12 0NN UK. Developmental Epigenetics University of Edinburgh Western General Hospital Edinburgh EH4 2XR UK. Current address Institute of Reproductive and Developmental Biology Imperial College School of Medicine London W12 0NN UK. Correspondence Helle F Jorgensen. Email . Amanda G Fisher. Email Published 17 August 2007 Genome Biology 2007 8 Rl69 doi gb-2007-8-8-rl 69 The electronic version of this article is the complete one and can be found online at http 2007 8 8 R169 Received 6 March 2007 Revised 26 June 2007 Accepted I7 August 2007 2007 Jorgensen et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The time of locus replication during S-phase is tightly regulated and correlates with chromatin state. Embryonic stem ES cells have an unusual chromatin profile where many developmental regulator genes that are not yet expressed are marked by both active and repressive histone modifications. This poised or bivalent state is also characterized by locus replication in early S-phase in ES cells while replication timing is delayed in cells with restricted developmental options. Results Here we used a panel of mutant mouse