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Báo cáo y học: " In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications. | Theoretical Biology and Medical Modelling BioMed Central Open Access In silico analysis of chimeric espA eae and tir fragments of Escherichia coli O157 H7 for oral immunogenic applications Jafar Amani1 2 S Latif Mousavi3 Sima Rafati4 and Ali H Salmanian 1 Address National Institute of Genetic Engineering and Biotechnology NIGEB Shahrak-e- Pajoohesh 15th Km Tehran -Karaj Highway Tehran IR Iran 2Baqiyatallah University of Medical Science Department of Biotechnology Tehran IR Iran 3Dept of Biology Faculty of Basic Sciences Shahed University Tehran IR Iran and 4Molecular Immunology and Vaccine Research Laboratory Dept. of Immunology Pasteur Institute of Iran Tehran IR Iran Email Jafar Amani - jamani@nigeb.ac.ir S Latif Mousavi - slmousavi@shahed.ac.ir Sima Rafati - sima-rafatisy@institute.pasteur.ac.ir Ali H Salmanian - salman@nigeb.ac.ir Corresponding author Published 8 December 2009 Received 18 July 2009 Theoretical Biology and Medical Modelling 2009 6 28 doi 10.1186 1742-4682-6-28 Accepted 8 December 2009 This article is available from http www.tbiomed.cOm content 6 1 28 2009 Amani et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In silico techniques are highly suited for both the discovery of new and development of existing vaccines. Enterohemorrhagic Escherichia coli O157 H7 EHEC exhibits a pattern of localized adherence to host cells with the formation of microcolonies and induces a specific histopathological lesion attaching effacing . The genes encoding the products responsible for this phenotype are clustered on a 35-kb pathogenicity island. Among these proteins Intimin Tir and EspA which are expressed by attaching-effacing genes are responsible for the attachment to epithelial cell that leads