tailieunhanh - báo cáo khoa học: " In silico identification of conserved microRNAs in large number of diverse plant species"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In silico identification of conserved microRNAs in large number of diverse plant species | BMC Plant Biology BioMed Central Research article In silico identification of conserved microRNAs in large number of diverse plant species Ramanjulu Sunkar and Guru Jagadeeswaran Open Access Address Department of Biochemistry and Molecular Biology Oklahoma State University Stillwater OK 74078 USA Email Ramanjulu Sunkar - Guru Jagadeeswaran - guruswa@ Corresponding author Published 16 April 2008 Received 14 December 2007 BMC Plant Biology 2008 8 37 doi 1471-2229-8-37 Accepted 16 April 2008 This article is available from http 1471-2229 8 37 2008 Sunkar and Jagadeeswaran licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background MicroRNAs miRNAs are recently discovered small non-coding RNAs that play pivotal roles in gene expression specifically at the post-transcriptional level in plants and animals. Identification of miRNAs in large number of diverse plant species is important to understand the evolution of miRNAs and miRNA-targeted gene regulations. Now-a-days publicly available databases play a central role in the in-silico biology. Because at least 21 miRNA families are conserved in higher plants a homology based search using these databases can help identify orthologs or paralogs in plants. Results We searched all publicly available nucleotide databases of genome survey sequences GSS high-throughput genomics sequences HTGS expressed sequenced tags ESTs and nonredundant NR nucleotides and identified 682 miRNAs in 155 diverse plant species. We found more than 15 conserved miRNA families in 11 plant species 10 to14 families in 10 plant species and 5 to 9 families in 29 plant species. Nineteen conserved miRNA families were identified in important

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