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Probiotic Lactobacillus Strains: in vitro and in vivo Studies

Institute of Fermentation Technology and Microbiology, Technical University, Lodz, Poland cDepartment of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, dInstitute of Biochemistry and Biophysics of Polish Academy of Sciences, Warsaw, Poland | Folia Microbiol. 54 6 533-537 2009 http www.biomed.cas.cz mbu folia Probiotic Lactobacillus Strains in vitro and in vivo Studies B. CUKROWSKAa I. MOTYLb H. KOZÁKOVÁc M. SCHWARZERc R.K. GÓRECKId E. KLEWICKAb K. SLIZEWSKAb Z. LIBUDZISZb Department of Pathology The Children s Memorial Health Institute 04-730 Warsaw Poland e-mail b.cukrowska@czd.pl Institute of Fermentation Technology and Microbiology Technical University Lodz Poland cDepartment of Immunology and Gnotobiology Institute of Microbiology Academy of Sciences of the Czech Republic Novy Hradek Czech Republic Institute of Biochemistry and Biophysics of Polish Academy of Sciences Warsaw Poland Received 16 July 2009 Revised version 19 November 2009 ABSTRACT. Three Lactobacillus strains LOCK 0900 LOCK 0908 LOCK 0919 out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb c mice affected cytokine TH1-TH2 balance toward nonallergic TH1 response. Spleen cells isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains produced significantly higher amounts of anti-allergic tumor necrosis factor-a and interferon-- than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood liver and spleen a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900 LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and or therapy of allergic diseases. Abbreviations CFU colony forming units Con A concanavalin A IFN interferon IL interleukin MLN mesenteric lymph nodes PBS phosphate-buffered saline TH T helper lymphocytes TGF transforming .