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Lecture Microbiology - Chapter 3: Microscopy and staining

Lecture Microbiology - Chapter 3: Microscopy and staining. This chapter presents the following content: Metric units, light properties (λ), light properties (RP), light travel, compound light microscopy, fluorescence microscopy,. | MICROSCOPY AND STAINING CHAPTER 3 Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Metric Units Microbe sizes – μm to nm Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Light Properties (λ) Wavelength – Electromagnetic spectrum – Shorter wavelength • Higher energy • More resolution Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Light Properties (RP) Resolving Power (Resolution) – Distance between 2 points that can be distinguished Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Light Travel Reflection – Waves are bounced off some materials Transmission – Waves pass through Absorption – Energy modification Copyright © 2012 John Wiley & Sons, Inc. All rights .