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Báo cáo y học: " Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells. | BioMed Central Retrovirology Research Effects of prostratin on Cyclin Tl P-TEFb function and the gene expression profile in primary resting CD4 T cells Tzu-Ling Sung and Andrew P Rice Open Access Address Department of Molecular Virology and Microbiology Baylor College of Medicine Houston Texas 77030 USA Email Tzu-Ling Sung - ts144315@bcm.tmc.edu Andrew P Rice - arice@bcm.tmc.edu Corresponding author Published 02 October 2006 Received 05 July 2006 Accepted 02 October 2006 Retrovirology 2006 3 66 doi l0.ll86 l 742-4690-3-66 This article is available from http www.retrovirology.cOm content 3 1 66 2006 Sung and Rice licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The latent reservoir of human immunodeficiency virus type 1 HIV-1 in resting CD4 T cells is a major obstacle to the clearance of infection by highly active antiretroviral therapy HAART . Recent studies have focused on searches for adjuvant therapies to activate this reservoir under conditions of HAART. Prostratin a non tumor-promoting phorbol ester is a candidate for such a strategy. Prostratin has been shown to reactivate latent HIV-1 and Tat-mediated transactivation may play an important role in this process. We examined resting CD4 T cells from healthy donors to determine if prostratin induces Cyclin T1 P-TEFb a cellular kinase composed of Cyclin T1 and Cyclin-dependent kinase-9 CDK9 that mediates Tat function. We also examined effects of prostratin on Cyclin T2a an alternative regulatory subunit for CDK9 and 7SK snRNA and the HEXIM1 protein two factors that associate with P-TEFb and repress its kinase activity. Results Prostratin up-regulated Cyclin T1 protein expression modestly induced CDK9 protein expression and did not affect Cyclin T2a .