tailieunhanh - Báo cáo y học: "Response to: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection – authors’ reply"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Response to: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection – authors’ reply. | Rutgers et al. Arthritis Research Therapy 2010 12 411 http content 12 6 411 LETTER Response to Cytokine profile of autologous conditioned serum for treatment of osteoarthritis in vitro effects on cartilage metabolism and intra-articular levels after injection - authors reply Marijn Rutgers1 Daniel BF Saris1 Wouter JA Dhert1-2 and Laura B Creemers 1 See related letter by Moser http content 12 6 410 and related research by Rutgers et al. http content 12 3 R114 We thank Moser for his comments 1 on the paper 2 we published in a previous issue of Arthritis Research Therapy. To dispel any concerns about the proper use of the product the autologous conditioned serum ACS in this study was prepared in a GMP good manufacturing practice facility in strict accordance with the guidelines supplied by the manufacturer Orthogen Dusseldorf Germany and was injected six times at 3-day intervals in accordance with the instructions given. As recommended immediately before injection of ACS synovial fluid was carefully aspirated to minimize ACS dilution. This synovial fluid was used to determine the cytokine levels before and during ACS treatment. We showed that 3 days after injection cytokine levels were at baseline and had no secondary effects on other cytokines. This finding is not in conflict with that of Darabos and colleagues 3 whose publication is cited by Moser upon careful reading of that publication none of the effects of ACS injection including the alleged decrease in synovial fluid levels of interleukin-1 IL-1 turned out to be statistically significant. We certainly agree with Moser that in vitro and in vivo studies should be well controlled. However in the case of ACS the use of an autologous product is not required this autologous product is devoid of cells which are the only factors capable of evoking an immune response upon transfer of human materials to human recipients. We have used unconditioned serum