tailieunhanh - Báo cáo y học: "Effects of ischemic pre- and postconditioning on HIF-1a, VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Effects of ischemic pre- and postconditioning on HIF-1a, VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver. | Knudsen et al. Comparative Hepatology 2011 10 3 http content 10 1 3 COMPARATIVE HEPATOLOGY RESEARCH Open Access Effects of ischemic pre- and postconditioning on HIF-1a VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver 1 1 3 1 1 Anders R Knudsen Anne-Sofie Kannerup Henning Gr0nb k Kasper J Andersen Peter Funch-Jensen Jan Frystyk2 Allan Flyvbjerg2 and Frank V Mortensen1 Abstract Background Ischemic pre- and postconditioning protects the liver against ischemia reperfusion injuries. The aim of the present study was to examine how ischemic pre- and postconditioning affects gene expression of hypoxia inducible factor 1a HIF-1a vascular endothelial growth factor A VEGF-A and transforming growth factor b TGF-b in liver tissue. Methods 28 rats were randomized into five groups control ischemia reperfusion ischemic preconditioning IPC ischemic postconditioning IPO combined IPC and IPO. IPC consisted of 10 min of ischemia and 10 min of reperfusion. IPO consisted of three cycles of 30 sec. reperfusion and 30 sec. of ischemia. Results HIF-1 a mRNA expression was significantly increased after liver ischemia compared to controls p . HIF-1a mRNA expression was significantly lower in groups subjected to IPC or combined IPC and IPO when compared to the ischemia reperfusion group p . VEGF-A mRNA expression increased in the ischemia reperfusion or combined IPC and IPO groups when compared to the control group p . Conclusion Ischemic conditioning seems to prevent HIF-1a mRNA induction in the rat liver after ischemia and reperfusion. This suggests that the protective effects of ischemic conditioning do not involve the HIF-1 system. On the other hand the magnitude of the HIF-1a response might be a marker for the degree of I R injuries after liver ischemia. Further studies are needed to clarify this issue. Background Colorectal cancer is a leading form of cancer in the Western world. Approximately 50 of patients .