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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes | Botticella et al. BMC Plant Biology 2011 11 156 http www.biomedcentral.com 1471-2229 11 156 BMC Plant Biology RESEARCH ARTICLE Open Access High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes 11 2 2 1 Ermelinda Botticella Francesco Sestili Antonio Hernandez-Lopez Andrew Phillips and Domenico Lafiandra Abstract Background Manipulation of the amylose-amylopectin ratio in cereal starch has been identified as a major target for the production of starches with novel functional properties. In wheat silencing of starch branching enzyme genes by a transgenic approach reportedly caused an increase of amylose content up to 70 of total starch exhibiting novel and interesting nutritional characteristics. In this work the functionality of starch branching enzyme IIa SBEIIa has been targeted in bread wheat by TILLING. An EMS-mutagenised wheat population has been screened using High Resolution Melting of PCR products to identify functional SNPs in the three homoeologous genes encoding the target enzyme in the hexaploid genome. Results This analysis resulted in the identification of 56 14 and 53 new allelic variants respectively for SBEIIa-A SBEIIa-B and SBEIIa-D. The effects of the mutations on protein structure and functionality were evaluated by a bioinformatic approach. Two putative null alleles containing non-sense or splice site mutations were identified for each of the three homoeologous SBEIIa genes qRT-PCR analysis showed a significant decrease of their gene expression and resulted in increased amylose content. Pyramiding of different single null homoeologous allowed to isolate double null mutants showing an increase of amylose content up to 21 compared to the control. Conclusion TILLING has successfully been used to generate novel alleles for SBEIIa genes known to control amylose content in wheat. Single and double null SBEIIa genotypes have been found to show a significant increase in amylose content. Background Reserve .