tailieunhanh - Báo cáo y học: "High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans."

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans. | Open Access High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans Arun K RamaniH Andrew C NelsonHn Philipp Kapranov Ian Bell Thomas R Gingeras and Andrew G Fraser Addresses Donnelly CCBR College Street University of Toronto Toronto M5S 3E1 Canada. The Wellcome Trust Sanger Institute Hinxton Cambridge CB10 1SA UK. Department of Physiology Development and Neuroscience University of Cambridge Cambridge CB2 3DY UK. Affymetrix Inc. Central Expressway Santa Clara CA 95051 USA. Helicos Biosciences Corporation Cambridge MA 02139 USA. Cold Spring Harbor Laboratory Cold Spring Harbor New York NY 11724 USA. H These authors contributed equally to this work. Correspondence Thomas R Gingeras. Email gingeras@. Andrew G Fraser. Email Published 24 September 2009 Genome Biology 2009 10 R101 doi gb-2009-10-9-r101 The electronic version of this article is the complete one and can be found online at http 2009 10 9 R101 Received 5 June 2009 Revised 1 1 August 2009 Accepted 24 September 2009 2009 Ramani et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background While many genome sequences are complete transcriptomes are less well characterized. We used both genome-scale tiling arrays and massively parallel sequencing to map the Caenorhabditis elegans transcriptome across development. We utilized this framework to identify transcriptome changes in animals lacking the nonsense-mediated decay NMD pathway. Results We find that while the majority of detectable transcripts map to known gene structures 5 of transcribed regions fall outside current gene annotations. We show that 40 of these are novel exons. Using both .