tailieunhanh - Báo cáo y học: " High-resolution transcription atlas of the mitotic cell cycle in budding yeast"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: High-resolution transcription atlas of the mitotic cell cycle in budding yeast. | Granovskaia et al. Genome Biology 2010 11 R24 http 2010 11 3 R24 Genome Biology RESEARCH Open Access High-resolution transcription atlas of the mitotie cell eyele in budding yeast m m z r l. A I -A 1 Y I irr I I r-i v-i mn1 2 c Di Fz l ỉ ZA3 4t I Sr S rr A I S S I I U-A Z I5 s l I i I A z t 6 r Daa r I 1 Marina V Granovskaia Lars J Jensen Mdnnew E Ritchie Joern loedling Ye Ning reel BorK Wolfgang Huber1 5 Lars M Steinmetz1 Abstract Background Extensive transcription of non-coding RNAs has been detected in eukaryotic genomes and is thought to constitute an additional layer in the regulation of gene expression. Despite this role their transcription through the cell cycle has not been studied genome-wide approaches have only focused on protein-coding genes. To explore the complex transcriptome architecture underlying the budding yeast cell cycle we used 8 bp tiling arrays to generate a 5 minute-resolution strand-specific expression atlas of the whole genome. Results We discovered 523 antisense transcripts of which 80 cycle or are located opposite periodically expressed mRNAs 135 unannotated intergenic non-coding RNAs of which 11 cycle and 109 cell-cycle-regulated proteincoding genes that had not previously been shown to cycle. We detected periodic expression coupling of sense and antisense transcript pairs including antisense transcripts opposite of Key cell-cycle regulators like FAR1 and TAF2. Conclusions Our dataset presents the most comprehensive resource to date on gene expression during the budding yeast cell cycle. It reveals periodic expression of both protein-coding and non-coding RNA and profiles the expression of non-annotated RNAs throughout the cell cycle for the first time. This data enables hypothesis-driven mechanistic studies concerning the functions of non-coding RNAs. Background Genome-wide transcriptome analyses in humans 1-5 mouse 6 Drosophila melanogaster 7 8 Arabidopsis thaliana 9 and fission and budding yeast 10-12 have .