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Báo cáo y học: " HIV-1 recombinants with multiple parental strains in low-prevalence, remote regions of Cameroon: Evolutionary relics?"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: " HIV-1 recombinants with multiple parental strains in low-prevalence, remote regions of Cameroon: Evolutionary relics. | Carr et al. Retrovirology 2010 7 39 http www.retrovirology.eom content 7 1 39 gtr RETR0VIR0L0GY RESEARCH Open Access HIV-1 recombinants with multiple parental strains in low-prevalence remote regions of Cameroon Evolutionary relics Jean KCarr 1 Nathan D Wolfe2 Judith N Torimiro3 4 Ubald Tamoufe5 E Mpoudi-Ngole5 Lindsay Eyzaguirre1 Deborah L Birx6 Francine E McCutchan7 and Donald S Burke8 Abstract Background The HIV pandemic disseminated globally from Central West Africa beginning in the second half of the twentieth century. To elucidate the virologic origins of the pandemic a cross-sectional study was conducted of the genetic diversity of HIV-1 strains in villagers in 14 remote locations in Cameroon and in hospitalized and STI patients. DNA extracted from PBMC was PCR amplified from HIV subjects. Partial pol amplicons N 164 and nearly full virus genomes N 78 were sequenced. Among the 3956 rural villagers studied the prevalence of HIV infection was 4.9 among the hospitalized and clinic patients it was 8.6 . Results Virus genotypes fell into two distinctive groups. A majority of the genotyped strains 109 164 were the circulating recombinant form CRF known to be endemic in West Africa and Central West Africa CRF02_AG. The second most common genetic form 9 164 was the recently described CRF22_01A1 and the rest were a collection of 4 different subtypes A2 D F2 G and 6 different CRFs -01 -11 -13 -18 -25 -37 . Remarkably 10.4 of HIV-1 genomes detected 17 164 were heretofore undescribed unique recombinant forms URF present in only a single person. Nearly full genome sequencing was completed for 78 of the viruses of interest. HIV genetic diversity was commonplace in rural villages 12 villages each had at least one newly detected URF and 9 villages had two or more. Conclusions These results show that while CRF02_AG dominated the HIV strains in the rural villages the remainder of the viruses had tremendous genetic diversity. Between the trans-species transmission of SIVcpz .