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Báo cáo y học: "Melatonin promoted chemotaxins expression in lung epithelial cell stimulated with TNF-α"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Melatonin promoted chemotaxins expression in lung epithelial cell stimulated with TNF-α. | Respiratory Research BioMed Central Research Open Access Melatonin promoted chemotaxins expression in lung epithelial cell stimulated with TNF-a FengMing Luo f XiaoJing Liuf ShuangQing Lit ChunTao Liu and ZengLi Wang Address West China Hospital of Sichuan University Chengdu China Email FengMing Luo - lufengming@hotmail.com XiaoJing Liu - liuxiaojing67@hotmail.com ShuangQing Li - shuangqli@hotmail.com ChunTao Liu - liuchuntao@163.net ZengLiWang - wangzengli@hotmail.com Corresponding author fEqual contributors Published 10 November 2004 Respiratory Research 2004 5 20 doi l0.ll86 l465-992l-5-20 Received 25 January 2004 Accepted l0 November 2004 This article is available from http respiratory-research.eom content 5 l 20 2004 Luo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Patients with asthma demonstrate circadian variations in the airway inflammation and lung function. Pinealectomy reduces the total inflammatory cell number in the asthmatic rat lung. We hypothesize that melatonin a circadian rhythm regulator may modulate the circadian inflammatory variations in asthma by stimulating the chemotaxins expression in the lung epithelial cell. Methods Lung epithelial cells A549 were stimulated with melatonin in the presence or absence of TNF-a l00 ng ml . RANTES Regulated on Activation Normal T-cells Expressed and Secreted and eotaxin expression were measured using ELISA and real-time RT-PCR eosinophil chemotactic activity ECA released by A549 was measured by eosinophil chemotaxis assay. Results TNF-a increased the expression of RANTES 307.84 33.56 versus 207.64 3l.27 pg ml of control p 0.025 and eotaxin l08.97 l0.87 versus 54.00 5.29 pg ml of control p 0.04l . Melatonin l0-l0 to l0-6M alone didn t .