tailieunhanh - Báo cáo y học: "Effects of saliva collection using cotton swabs on melatonin enzyme immunoassay"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Effects of saliva collection using cotton swabs on melatonin enzyme immunoassay. | Kozaki et al. Journal of Circadian Rhythms 2011 9 1 http content 9 1 1 JOURNAL OF CIRCADIAN RHYTHMS SHORT PAPER Open Access Effects of saliva collection using cotton swabs on melatonin enzyme immunoassay Tomoaki Kozaki1 Soomin Lee2 Takayuki Nishimura3 Tetsuo Katsuura2 Akira Yasukouchi4 Abstract Background Although various acceptable and easy-to-use devices have been used for saliva collection cotton swabs are among the most common ones. Previous studies reported that cotton swabs yield a lower level of melatonin detection. However this statistical method is not adequate for detecting an agreement between cotton saliva collection and passive saliva collection and a test for bias is needed. Furthermore the effects of cotton swabs have not been examined at lower melatonin level a level at which melatonin is used for assessment of circadian rhythms namely dim light melatonin onset DLMO . In the present study we estimated the effect of cotton swabs on the results of salivary melatonin assay using the Bland-Altman plot at lower level. Methods Nine healthy males were recruited and each provided four saliva samples on a single day to yield a total of 36 samples. Saliva samples were directly collected in plastic tubes using plastic straws and subsequently pipetted onto cotton swabs cotton saliva collection and into clear sterile tubes passive saliva collection . The melatonin levels were analyzed in duplicate using commercially available ELISA kits. Results The mean melatonin concentration in cotton saliva collection samples was significantly lower than that in passive saliva collection samples at higher melatonin level 6 pg mL . The Bland-Altman plot indicated that cotton swabs causes relative and proportional biases in the assay results. For lower melatonin level 6 pg mL although the BA plots didn t show proportional and relative biases there was no significant correlation between passive and cotton saliva collection samples. Conclusion Our .