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Báo cáo y học: " amplification refractory mutation system polymerase chain reaction assay: studies in patients with asthma"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: amplification refractory mutation system polymerase chain reaction assay: studies in patients with asthma. | Available online http respiratory-research.eom content 2 4 255 Primary research Simultaneous identification of GSTP1 Ile105 Val105 and Ala114 Val114 substitutions using an amplification refractory mutation system polymerase chain reaction assay studies in patients with asthma Anja Hemmingsen Anthony A Fryer Michael Hepple Richard C Strange and Monica A Spiteri Centre for Cell Molecular Medicine Keele University North Staffordshire Hospital Staffordshire England Correspondence Anja Hemmingsen Centre for Cell Molecular Medicine Keele University North Staffordshire Hospital Staffordshire ST4 6QG UK. Tel 44 1782 554 765 fax 44 1782 552 323 e-mail med06@cc.keele.ac.uk Received 18 January 2001 Revisions requested 27 February 2001 Revisions received 5 April 2001 Accepted 10 May 2001 Published 11 June 2001 Respir Res 2001 2 255-260 2001 Hemmingsen et al licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background The glutathione S-transferase GST enzyme GSTP1 utilizes byproducts of oxidative stress. We previously showed that alleles of GSTP1 that encode the Ile105 Val105 substitution are associated with the asthma phenotypes of atopy and bronchial hyperresponsiveness BHR . However a further polymorphic site Ala114 Val114 has been identified that results in the following alleles GSTP1 A wild-type Ile105 Ala114 GSTP1 B Val105 Ala114 GSTP1 C Val1054val114 and GSTP1 D JleÌ05 Val114 . Methods Because full identification of GSTP1 alleles may identify stronger links with asthma phenotypes we describe an amplification refractory mutation system ARMS assay that allows identification of all genotypes. We explored whether the GSTP1 substitutions influence susceptibility to asthma atopy and BHR. Results Among 191 atopic nonasthmatic atopic asthmatic and nonatopic nonasthmatic individuals none had the BD CD or DD genotypes. GSTP1 BC was significantly associated with reduced risk for atopy P 0.031 . Compared with AA trend test analysis identified a .