tailieunhanh - Báo cáo y học: "Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy. | Open Access Modulation of gene expression in drug resistant Leishmania is associated with gene amplification gene deletion and chromosome aneuploidy Jean-Michel Ubeda Danielle Légaré Frédéric Raymond Amin Ahmed Ouameur Sébastien Boisvert Philippe Rigault Jacques Corbeil Michel J Tremblay Martin Olivier Barbara Papadopoulou and Marc Ouellette Addresses Université Laval Division de Microbiologie Centre de Recherche en Infectiologie boulevard Laurier Québec G1V 4G2 Canada. tUniversité Laval Centre de Recherche en Endocrinologie Moléculaire et Oncologique boulevard Laurier Québec G1V 4G2 Canada. McGill University Department of Microbiology and Immunology Lyman Duff Medical Building University Street Montreal H3A 2B4 Canada. Correspondence Marc Ouellette. Email Published 18 July 2008 Genome Biology 2008 9 R115 doi gb-2008-9-7-r1 15 The electronic version of this article is the complete one and can be found online at http content 9 7 R115 Received 25 February 2008 Revised 6 June 2008 Accepted 18 July 2008 2008 Ubeda et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Drug resistance can be complex and several mutations responsible for it can coexist in a resistant cell. Transcriptional profiling is ideally suited for studying complex resistance genotypes and has the potential to lead to novel discoveries. We generated full genome 70-mer oligonucleotide microarrays for all protein coding genes of the human protozoan parasites Leishmania major and Leishmania infantum. These arrays were used to monitor gene expression in methotrexate resistant parasites. Results Leishmania is a eukaryotic organism with minimal control at the level of transcription .