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Báo cáo y học: "Whole blood transcriptional profiling in ankylosing spondylitis identifies novel candidate genes that might contribute to the inflammatory and tissue-destructive disease aspects"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Whole blood transcriptional profiling in ankylosing spondylitis identifies novel candidate genes that might contribute to the inflammatory and tissue-destructive disease aspects. | Pimentel-Santos et al. Arthritis Research Therapy 2011 13 R57 http arthritis-research.eom content 13 2 R57 RESEARCH ARTICLE Open Access Whole blood transcriptional profiling in ankylosing spondylitis identifies novel candidate genes that might contribute to the inflammatory and tissue-destructive disease aspects rarn nrl i K l DimonPol-CTỉnfmc1 2 3 6rim I inoirr i4 Irlsj h l fmc5 AfiTi p ir5m1 3 Ir iCQ r mef 6 I lolonTỉ C-s y- .-f-f ir7 reiiidiiuu M Pimentel Sdntos Ddrio Ligeiro Mdidldd Ividtos And F Mourdo José Costd Helend Sdntos 8 9 10 11 12.13 Andbeld Bdrcelos Fdtimd Godinho Pdtricid Pinto Mdrgdridd Cruz Judo E Fonsecd Henrique Guedes-Pinto2 Jdime C Brdnco1 3 Mdtthew A Brown14 dnd Gethin P Thomds14 Abstract Introduction A number of genetic-dssocidtion studies hdve identified genes contributing to dnkylosing spondylitis AS susceptibility but such dpprodches provide little informdtion ds to the gene dctivity chdnges occurring during the disedse process. Trdnscriptiondl profiling generates d sndpshot of the sdmpled cells dctivity dnd thus cdn provide insights into the moleculdr processes driving the disedse process. We undertook d whole-genome microdrrdy dpprodch to identify cdndiddte genes dssocidted with AS dnd vdliddted these gene-expression chdnges in d ldrger sdmple cohort. Methods A totdl of 18 dctive AS pdtients cldssified dccording to the New York criterid dnd 18 gender- dnd dge-mdtched controls were profiled using Illuming HT-12 whole-genome expression BeddChips which cdrry cDNAs for 48 000 genes dnd transcripts. Cldss compdrison dndlysis identified d number of differentidlly expressed cdndiddte genes. These cdndiddte genes were then vdliddted in d ldrger cohort using qPCR-bdsed TdqMdn low density drrdys TLDAs . Results A totdl of 239 probes corresponding to 221 genes were identified ds being significdntly different between pdtients dnd controls with d P-vdlue 0.0005 80 confidence level of fdlse discovery rdte . Forty-seven genes were then selected for .