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Báo cáo y học: "TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis. | Dharmapatni et al. Arthritis Research Therapy 2011 13 R51 http arthritis-research.eom content 13 2 R51 RESEARCH ARTICLE Open Access TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis 1 2 1 1 s . 4 Anak ASSK Dharmapatni Malcolm D Smith Tania N Crotti Christopher A Holding Cristina Vincent I lolon KA yA ooHf ir i2 AnHro A A f if iCi1 l if i 3 6 c 7bcitin4 rh7 ih KA PinHlsỉx 5 6 I Ai lzinc5 6t r iA Helen M Weedon Andrew CW zannettino Timothy S Zheng David M Findlay Geiald J Atkins and David R Haynes1t Abstract Introduction TNF-like weak inducer of apoptosis TWEAK has been proposed as a mediator of inflammation and bone erosion in rheumatoid arthritis RA . This study aimed to investigate TWEAK and TWEAK receptor Fn14 expression in synovial tissue from patients with active and inactive rheumatoid arthritis RA osteoarthritis OA and normal controls and assess soluble s TWEAK levels in the synovial fluids from patients with active RA and OA. Effects of sTWEAK on osteoclasts and osteoblasts were investigated in vitro. Methods TWEAK and Fn14 expression were detected in synovial tissues by immunohistochemistry IHC . Selected tissues were dual labelled with antibodies specific for TWEAK and lineage-selective cell surface markers CD68 Tryptase G CD22 and CD38. TWEAK mRNA expression was examined in human peripheral blood mononuclear cells PBMC sorted on the basis of their expression of CD22. sTWEAK was detected in synovial fluid from OA and RA patients by ELISA. The effect of sTWEAK on PBMC and RAW 264.7 osteoclastogenesis was examined. The effect of sTWEAK on cell surface receptor activator of NF Kappa B Ligand RANKL expression by human osteoblasts was determined by flow cytometry. Results TWEAK and Fn14 expression were significantly higher in synovial tissue from all patient groups compared to the synovial tissue from control subjects P 0.05 . TWEAK was significantly higher in active compared with inactive RA tissues P