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Báo cáo y học: "Anti-class a scavenger receptor autoantibodies from systemic lupus erythematosus patients impair phagocytic clearance of apoptotic cells by macrophages in vitro"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Anti-class a scavenger receptor autoantibodies from systemic lupus erythematosus patients impair phagocytic clearance of apoptotic cells by macrophages in vitro. | Chen et al. Arthritis Research Therapy 2011 13 R9 http arthritis-research.eom content 13 1 R9 RESEARCH ARTICLE Open Access Anti-class a scavenger receptor autoantibodies from systemic lupus erythematosus patients impair phagocytic clearance of apoptotic cells by macrophages in vitro Xiao-wei Chen1 2t Yan ShenH Chuan-yin Sun1 Feng-xia Wu1 Yi Chen1 Cheng-de Yang1 Abstract Introduction Inadequate clearance of apoptotic cells by macrophages is one of the reasons for the breakdown of self-tolerance. Class A scavenger receptors macrophage receptor with collagenous structure MARCO and scavenger receptor A SR-A which are expressed on macrophages play important roles in the uptake of apoptotic cells. A previous study reported the presence of the anti-MARCO antibody in lupus-prone mice and systemic lupus erythematosus SLE patients. The purpose of this study was to investigate the prevalence of anti-class A scavenger receptor antibodies in patients with various autoimmune diseases in particular SLE and the functional implication of those autoantibodies in the phagocytic clearance of apoptotic cells by macrophages. Methods Purified recombinant scavenger receptor cysteine-rich SRCR polypeptide ligand-binding domain of MARCO and recombinant SR-A were used as antigens. By using enzyme-linked immunosorbent assay the anti-SRCR and anti-SR-A antibodies were detected in the sera of untreated patients with SLE n 65 rheumatoid arthritis n 65 primary Sjogren syndrome n 25 and healthy blood donors n 85 . The effect of IgG purified from SLE patients or healthy controls on the phagocytosis of apoptotic cells by macrophages was measured by the flow cytometry assay. Results Anti-SRCR antibodies were present in patients with SLE 18.5 and rheumatoid arthritis 3.1 but not in those with primary Sjogren syndrome. Anti-SR-A antibodies were present in patients with SLE 33.8 rheumatoid arthritis 13.8 and primary Sjogren syndrome 12.0 . IgG from SLE patients positive for anti-SRCR or anti-SR-A .