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Báo cáo y học: "Extracorporeal immune therapy with immobilized agonistic anti-Fas antibodies leads to transient reduction of circulating neutrophil numbers and limits tissue damage after hemorrhagic shock/resuscitation in a porcine model"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Extracorporeal immune therapy with immobilized agonistic anti-Fas antibodies leads to transient reduction of circulating neutrophil numbers and limits tissue damage after hemorrhagic shock/resuscitation in a porcine model. | Logters et al. Journal of Inflammation 2010 7 18 http www.journal-inflammation.eom content 7 1 18 JOURNAL OF INFLAMMATION RESEARCH Open Access Extracorporeal immune therapy with immobilized agonistic anti-Fas antibodies leads to transient reduction of circulating neutrophil numbers and limits tissue damage after hemorrhagic shock resuscitation in a porcine model Tim T Lõgters 1 2 Jens Altrichter1 Adnana Paunel-Gorgulu1 Martin Sager1 Ingo Witte1 Annina Ott1 Sarah Sadek1 Jessica Baltes1 José Bitu-Moreno3 Alberto Schek1 Wolfram Muller4 Teresa Jeri1 Joachim Windolf1 and Martin Scholz1 Abstract Background Hemorrhagic shock resuscitation is associated with aberrant neutrophil activation and organ failure. This experimental porcine study was done to evaluate the effects of Fas-directed extracorporeal immune therapy with a leukocyte inhibition module LIM on hemodynamics neutrophil tissue infiltration and tissue damage after hemorrhagic shock resuscitation. Methods In a prospective controlled double-armed animal trial 24 Munich Mini Pigs 30.3 3.3 kg were rapidly haemorrhaged to reach a mean arterial pressure MAP of 35 5 mmHg maintained hypotensive for 45 minutes and then were resuscitated with Ringer solution to baseline MAP With beginning of resuscitation 12 pigs underwent extracorporeal immune therapy for 3 hours LIM group and 12 pigs were resuscitated according to standard medical care SMC . Haemodynamics haematologic metabolic and organ specific damage parameters were monitored. Neutrophil infiltration was analyzed histologically after 48 and 72 hours. Lipid peroxidation and apoptosis were specifically determined in lung bowel and liver. Results In the LIM group neutrophil counts were reduced versus SMC during extracorporeal immune therapy. After 72 hours the haemodynamic parameters MAP and cardiac output CO were significantly better in the LIM group. Histological analyses showed reduction of shock-related neutrophil tissue infiltration in the LIM group especially in .