Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo y học: "The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease. | Available online http arthritis-research.eom content 10 2 R46 Research article The influence of serum glucose and oxygen on intervertebral disc cell growth in vitro implications for degenerative disc disease William EB Johnson1 2 Simon Stephan1 2 and Sally Roberts1 2 Open Access Centre for Spinal Studies Robert Jones and Agnes Hunt Orthopaedic Hospital Oswestry Shropshire SY10 7AG UK institute for Science and Technology in Medicine Keele University Keele Staffordshire ST5 5BG UK Corresponding author William EB Johnson Eustace.Johnson@rjah.nhs.uk Received 14 Jan 2008 Revisions requested 7 Mar 2008 Revisions received 2 Apr 2008 Accepted 23 Apr 2008 Published 23 Apr 2008 Arthritis Research Therapy 2008 10 R46 doi 10.1186 ar2405 This article is online at http arthritis-research.com content 10 2 R46 2008 Johnson et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction The avascular nature of the human intervertebral disc IVD is thought to play a major role in disc pathophysiology by limiting nutrient supply to resident IVD cells. In the human IVD the central IVD cells at maturity are normally chondrocytic in phenotype. However abnormal cell phenotypes have been associated with degenerative disc diseases including cell proliferation and cluster formation cell death stellate morphologies and cell senescence. Therefore we have examined the relative influence of possible blood-borne factors on the growth characteristics of IVD cells in vitro. Methods Bovine IVD cells were cultured either in monolayer to encourage cell proliferation or in alginate to induce chondrocytic differentiation. In both culture systems cells were maintained with or without 20 serum with or without 320 mg dL glucose and in atmospheric .