Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo y học: "SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis. | Available online http arthritis-research.eom content 9 5 R107 Open Access Research article SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis Simon R Tew1 Peter D Clegg1 2 Christopher J Brew1 Colette M Redmond2 and Timothy E Hardingham1 1 UK Centre for Tissue Engineering Wellcome Trust Centre for Cell-Matrix Research Faculty of Life Sciences University of Manchester Michael Smith Building Oxford Road Manchester M13 9PT UK 2Faculty of Veterinary Sciences University of Liverpool Leahurst Neston CH64 7TE UK Corresponding author Timothy E Hardingham timothy.e.hardingham@manchester.ac.uk Received 10 Aug 2007 Revisions requested 30 Aug 2007 Revisions received 26 Sep 2007 Accepted 12 Oct 2007 Published 12 Oct 2007 Arthritis Research Therapy 2007 9 R107 doi 10.1186 ar2311 This article is online at http arthritis-research.com content 9 5 R107 2007 Tew et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The transcription factor SOX9 is important in maintaining the chondrocyte phenotype. To identify novel genes regulated by SOX9 we investigated changes in gene expression by microarray analysis following retroviral transduction with SOX9 of a human chondrocytic cell line SW1353 . From the results the expression of a group of genes SRPX S100A1 APOD RGC32 CRTL1 MYBPH CRLF1 and SPINT1 was evaluated further in human articular chondrocytes HACs . First the same genes were investigated in primary cultures of HACs following SOX9 transduction and four were found to be similarly regulated SRPX APOD CRTL1 and S100A1 . Second during dedifferentiation of HACs by passage in monolayer cell culture during which the expression of SOX9 progressively .