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Báo cáo y học: "Abnormal insulin-like growth factor 1 signaling in human osteoarthritic subchondral bone osteoblasts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Abnormal insulin-like growth factor 1 signaling in human osteoarthritic subchondral bone osteoblasts. | Available online http arthritis-research.eom content 8 6 R1 77 Open Access Research article Abnormal insulin-like growth factor 1 signaling in human osteoarthritic subchondral bone osteoblasts Frédéric Massicotte1 Isabelle Aubry1 Johanne Martel-Pelletier1 Jean-Pierre Pelletier1 Julio Fernandes2 and Daniel Lajeunesse1 1Unité de recherche en arthrose Centre de recherche du centre hospitalier de l Université de Montréal Hôpital Notre-Dame Montréal Québec Canada 2Centre de recherche Hôpital Sacré-Creur Montréal Québec Canada Corresponding author Daniel Lajeunesse daniel.lajeunesse@umontreal.ca Received 20 Sep 2006 Revisions requested 29 Sep 2006 Revisions received 20 Oct 2006 Accepted 27 Nov 2006 Published 27 Nov 2006 Arthritis Research Therapy 2006 8 R177 doi 10.1186 ar2087 This article is online at http arthritis-research.com content 8 6 R1 77 2006 Massicotte et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Insulin-like growth factor IGF -1 is a key factor in bone homeostasis and could be involved in bone tissue sclerosis as observed in osteoarthritis OA . Here we compare the key signaling pathways triggered in response to IGF-1 stimulation between normal and OA osteoblasts Obs . Primary Obs were prepared from the subchondral bone of tibial plateaus of OA patients undergoing knee replacement or from normal individuals at autopsy. Phenotypic characterization of Obs was evaluated with alkaline phosphatase and osteocalcin release. The effect of IGF-1 on cell proliferation alkaline phosphatase and collagen synthesis was evaluated in the presence or not of 50 ng ml IGF-1 whereas signaling was studied with proteins separated by SDS-PAGE before western blot analysis. We also used immunoprecipitation followed by .