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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis B virus"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis B virus. | Int. J. Med. Sci. 2005 2 1 30 Research paper Received 2004.11.01 Accepted 2005.01.01 PubliShed 2005.01.05 Abstract Key words Author biography Corresponding address International Journal of Medical Sciences ISSN 1449-1907 www.medsci.org 2005 2 1 30-35 2005 Ivyspring International Publisher. All rights reserved High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis B virus Xuanyong Lu Matthew Lee Trang Tran and Timothy Block Drexel Institute for Biotechnology and Virology Research Department of Microbiology and Immunology School of Medicine Drexel University Doylestown PA 18901 USA The serious result of hepatitis B HBV virus infection is development of hepatocellular carcinoma HCC . However the reason of development of HCC in HBV infected patients is still unclear. Recently the suppression of cell apoptosis is found to relate with the development of cell carcinogenesis therefore the expression of apoptosis inhibitor in the virus related cancer line such as hepatoma cell line HepG2.2i5 was investigated. There are at least six Human apoptosis inhibitors IAP have been identified now. They are clAPi cIAP2 XIAP NAPI survivin and plAP. Using gene-assay technology we have recently compared the expression of IAPs in the HepG2.2i5 cells that persistently expresses Hepatitis B virus by integrated HBV genome with its parent cell line HepG2. The results suggest that there was obviously increase of cIAP2 and cIAPi in the HepG2.2i5 cells versus HepG2 cells. Those observations imply a possibility of long time HBV infection could induce the over-expressing apoptosis inhibitors furthermore causing the liver cancer. The high expression of cIAPi and cIAP2 in HBV expressing cells was confirmed by RT-PCR and Northern blot analysis. However we did not find the change of NIAP and suvivin in HepG2.2i5 cells. In contrast the expression of XIAP was down in the HepG2.2i5 cells comparing with HepG2 cells. How HBV triggers the over-expression of apoptosis .