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Báo cáo y học: "Insight into the molecular requirements for pathogenicity of Fusarium oxysporum"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Insight into the molecular requirements for pathogenicity of Fusarium oxysporum. | Open Access Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f. sp. lycopersici through large-scale insertional mutagenesis Caroline B MichielseH Ringo van WijkHt Linda Reijnen Ben JC Cornelissen and Martijn Rep Addresses Plant Pathology Swammerdam Institute for Life Sciences University of Amsterdam Kruislaan 318 1098 SM Amsterdam The Netherlands. Current address Plant Physiology Swammerdam Institute for Life Sciences University of Amsterdam Kruislaan 318 1098 SM Amsterdam The Netherlands. H These authors contributed equally to this work. Correspondence Caroline B Michielse. Email c.b.michielse@uva.nl Published 9 January 2009 Genome Biology 2009 10 R4 doi 10.ll86 gb-2009-l 0-l-r4 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 10 l R4 Received l October 2008 Revised 22 December 2008 Accepted 9 January 2009 2009 Michielse et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Fusarium oxysporum f. sp. lycopersici is the causal agent of vascular wilt disease in tomato. In order to gain more insight into the molecular processes in F. oxysporum necessary for pathogenesis and to uncover the genes involved we used Agrobacterium-mediated insertional mutagenesis to generate l0 290 transformants and screened the transformants for loss or reduction of pathogenicity. Results This led to the identification of 106 pathogenicity mutants. Southern analysis revealed that the average T-DNA insertion is l .4 and that 66 of the mutants carry a single T-DNA. Using TAIL-PCR chromosomal T-DNA flanking regions were isolated and lll potential pathogenicity genes were identified. Conclusions Functional categorization of the potential