tailieunhanh - Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning. | Open Access Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2 lineage cells an insight into mesodermal patterning Michael Xavier Doss Shuhua Chen Johannes Winkler Rita Hippler-Altenburg Margareta Odenthal Claudia Wickenhauser Sridevi Balaraman Herbert Schulz Oliver Hummel Norbert Hubner Nandini Ghosh-Choudhury Isaia Sotiriadou Jurgen Hescheler and Agapios Sachinidis Addresses institute of Neurophysiology University of Cologne Robert-Koch Str. 39 50931 Cologne Germany. Tnstitute of Pathology University of Cologne Joseph-Stelzmann-Str. 9 50931 Cologne Germany. Max-Delbrueck-Center for Molecular Medicine - MDC Robert-Rossle Str. 10 13092 Berlin Germany. Department of Pathology The University of Texas Health Science Center at San Antonio TX 78229 USA. Correspondence Agapios Sachinidis. Email Published 4 September 2007 Genome Biology 2007 8 R184 doi gb-2007-8-9-rl 84 The electronic version of this article is the complete one and can be found online at http 2007 8 9 R184 Received 11 December 2006 Revised 30 May 2007 Accepted 4 September 2007 2007 Doss et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Bone morphogenetic protein BMP 2 is a late mesodermal marker expressed during vertebrate development and plays a crucial role in early embryonic development. The nature of the BMP2-expressing cells during the early stages of embryonic development their transcriptome and cell phenotypes developed from these cells have not yet been characterized. Results We generated a transgenic BMP2 embryonic stem ES cell lineage expressing both puromycin acetyltransferase and enhanced green fluorescent protein EGFP driven by the BMP2 .