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Báo cáo hóa học: "Expression of Msx-1 is suppressed in bisphosphonate associated osteonecrosis related jaw tissue-etiopathology considerations respecting jaw developmental biology-related unique features"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Expression of Msx-1 is suppressed in bisphosphonate associated osteonecrosis related jaw tissue-etiopathology considerations respecting jaw developmental biology-related unique features | Wehrhan et al. Journal of Translational Medicine 2010 8 96 http www.translational-medicine.eom content 8 1 96 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Expression of Msx-1 is suppressed in bisphosphonate associated osteonecrosis related jaw tissue-etiopathology considerations respecting jaw developmental biology-related unique features 1 2 1 1 1 1 Falk Wehrhan Peter Hyckel Jutta Ries Phillip Stockmann Emeka Nkenke Karl A Schlegel Friedrich W Neukam1 Kerstin Amann3 Abstract Background Bone-destructive disease treatments include bisphosphonates and antibodies against the osteoclast differentiator RANKL aRANKL however osteonecrosis of the jaw ONJ is a frequent side-effect. Current models fail to explain the restriction of bisphosphonate BP -related and denosumab anti-RANKL antibody -related ONJ to jaws. Msx-1 is exclusively expressed in craniofacial structures and pivotal to cranial neural crest CNC -derived periodontal tissue remodeling. We hypothesised that Msx-1 expression might be impaired in bisphosphonate-related ONJ. The study aim was to elucidate Msx-1 and RANKL-associated signal transduction BMP-2 4 RANKL in ONJ-altered and healthy periodontal tissue. Methods Twenty ONJ and twenty non-BP exposed periodontal samples were processed for RT-PCR and immunohistochemistry. An automated staining-based alkaline phosphatase-anti-alkaline phosphatase method was used to measure the stained cells total cell-number ratio labelling index Bonferroni adjustment . Real-time RT-PCR was performed on ONJ-affected and healthy jaw periodontal samples n 20 each to quantitatively compare Msx-1 BMP-2 RANKL and GAPDH mRNA levels. Results Semi-quantitative assessment of the ratio of stained cells showed decreased Msx-1 and RANKL and increased BMP-2 4 all p 0.05 expression in ONJ-adjacent periodontal tissue. ONJ tissue also exhibited decreased relative gene expression for Msx-1 p 0.03 and RANKL p 0.03 and increased BMP-2 4 expression p 0.02 compared to control. Conclusions .