tailieunhanh - Báo cáo hóa học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps"

Tham khảo tài liệu 'báo cáo hóa học: " expression of rna virus proteins by rna polymerase ii dependent expression plasmids is hindered at multiple steps"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps Nicola Ternette Daniela Stefanou Seraphin Kuate Klaus uberla and Thomas Grunwald Address Department of Molecular and Medical Virology Ruhr-Universitat Bochum 44780 Bochum Germany Email NicolaTernette - Daniela Stefanou - Seraphin Kuate - sekuate@ Klaus uberla - Thomas Grunwald - Corresponding author Published 5 June 2007 Received 8 March 2007 Accepted 5 June 2007 Virology Journal 2007 4 51 doi 1743-422X-4-51 This article is available from http content 4 1 51 2007 Ternette et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Proteins of human and animal viruses are frequently expressed from RNA polymerase II dependent expression cassettes to study protein function and to develop gene-based vaccines. Initial attempts to express the G protein of vesicular stomatitis virus VSV and the F protein of respiratory syncytial virus RSV by eukaryotic promoters revealed restrictions at several steps of gene expression. Results Insertion of an intron flanked by exonic sequences 5 -terminal to the open reading frames ORF of VSV-G and RSV-F led to detectable cytoplasmic mRNA levels of both genes. While the exonic sequences were sufficient to stabilise the VSV-G mRNA cytoplasmic mRNA levels of RSV-F were dependent on the presence of a functional intron. Cytoplasmic VSV-G mRNA levels led to readily detectable levels of VSV-G protein whereas RSV-F protein expression remained undetectable. However RSV-F expression was observed after .