tailieunhanh - báo cáo hóa học:" A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium | Sakamoto et al. AMB Express 2012 2 7 http content 2 1 7 o AMB Express a SpringerOpen Journal ORIGINAL Open Access A calmodulin inhibitor W-7 influences the effect of cyclic adenosine 3 5 -monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium 11 12 2 1 Takaiku Sakamoto Yuki Yao Yoshifumi Hida Yoichi Honda Takashi Watanabe Wataru Hashigaya Kazumi Suzuki 1 and Toshikazu Irie1 Abstract The capacity of white-rot fungi to degrade wood lignin may be highly applicable to the development of novel bioreactor systems but the mechanisms underlying this function are not yet fully understood. Lignin peroxidase LiP and manganese peroxidase MnP which are thought to be very important for the ligninolytic property demonstrated increased activity in Phanerochaete chrysosporium RP-78 FGSC 9002 ATCC MYA-4764 cultures following exposure to 5 mM cyclic adenosine 3 5 -monophosphate cAMP and 500 gM 3 -isobutyl-1-methylxanthine IBMX a phosphodiesterase inhibitor. Real-time reverse transcription polymerase chain reaction RT-PCR analysis revealed that transcription of most LiP and MnP isozyme genes was statistically significantly upregulated in the presence of the cAMP and IBMX compared to the untreated condition. However 100 gM calmodulin CaM inhibitor N- 6-aminohexyl -5-chloro-1-naphthalenesulfonamide W-7 which had insignificant effects on fungal growth and intracellular cAMP concentration not only offset the increased activity and transcription induced by the drugs but also decreased them to below basal levels. Like the isozyme genes transcription of the CaM gene cam was also upregulated by cAMP and IBMX. These results suggest that cAMP signaling functions to increase the transcription of LiP and MnP through the induction of cam transcription. Keywords Phanerochaete chrysosporium cAMP signaling Calmodulin signaling Lignin peroxidase Manganese peroxidase Introduction White-rot fungi are known to have a powerful ligninoly-tic system