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Báo cáo y học: "High-throughput analysis of spatio-temporal dynamics in Dictyostelium"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: High-throughput analysis of spatio-temporal dynamics in Dictyostelium. | Open Access Method High-throughput analysis of spatio-temporal dynamics in Dictyostelium Satoshi Sawai Xiao-Juan Guan Adam Kuspa and Edward C Cox Addresses Department of Molecular Biology Princeton University Princeton NJ 08544 USA. TRATO Complex Systems Biology Project JST Tokyo 153-8902 Japan. Departments of Biochemistry and Molecular and Human Genetics Baylor College of Medicine Houston TX 77030 USA. Correspondence Satoshi Sawai. Email ssawai@complex.c.u-tokyo.ac.jp Published 21 July 2007 Genome Biology 2007 8 R144 doi 10.1186 gb-2007-8-7-r144 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 7 R144 Received 17 April 2007 Revised 25 June 2007 Accepted 21 July 2007 2007 Sawai et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2 000 mutant clones from a large mutagenesis collection. Approximately 4 of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis. Background Spatially and temporally evolving collective dynamics act critically to coordinate multicellular development. In general periodic phenomena are prevalent in transcriptional regulation - for example in circadian rhythms 1 Msn transcription factor regulation in yeast 2 and the pulsatile response of NF-kB and p53 in tissue culture cells following stimulation 3 4 . Oscillations seem to be a universal