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Báo cáo y học: "Comprehensive characterization of the cis-regulatory code responsible for the spatio-temporal expression of olSix3.2 in the developing medaka forebrain"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Comprehensive characterization of the cis-regulatory code responsible for the spatio-temporal expression of olSix3.2 in the developing medaka forebrain. | Open Access Research Comprehensive characterization of the cis-regulatory code responsible for the spatio-temporal expression of olSix3.2 in the developing medaka forebrain Ivan Conte and Paola Bovolenta Address Departamento de Neurobiología Celular Molecular y del Desarrollo Instituto Cajal CSIC Dr Arce Madrid 28002 Spain. Correspondence Paola Bovolenta. Email bovolenta@cajal.csic.es Published 6 July 2007 Genome Biology 2007 8 R137 doi 10.1186 gb-2007-8-7-r137 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 7 R137 Received 23 February 2007 Revised 5 June 2007 Accepted 6 July 2007 2007 Conte and Bovolenta. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Embryonic development is coordinated by sets of c s-regulatory elements that are collectively responsible for the precise spatio-temporal organization of regulatory gene networks. There is little information on how these elements which are often associated with highly conserved noncoding sequences are combined to generate precise gene expression patterns in vertebrates. To address this issue we have focused on S x3 an important regulator of vertebrate forebrain development. Results Using computational analysis and exploiting the diversity of teleost genomes we identified a cluster of highly conserved noncoding sequences surrounding the S x3 gene. Transgenesis in medaka fish demonstrates that these sequences have enhancer silencer and silencer blocker activities that are differentially combined to control the entire distribution of S x3. Conclusion This report provides the first example of the precise regulatory code necessary for the expression of a vertebrate gene and offers a unique .