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Báo cáo khoa học: Modulation of the endocannabinoid system by focal brain ischemia in the rat is involved in neuroprotection afforded by 17b-estradiol

Endogenous levels of the endocannabinoid anandamide, and the activities of the synthesizing and hydrolyzing enzymes, i.e.N-acylphosphatidyletha-nolamine-hydrolyzing phospholipase D and fatty acid amide hydrolase, respectively, were determined in the cortex and the striatum of rats sub-jected to transient middle cerebral artery occlusion. | ễFEBS Journal Modulation of the endocannabinoid system by focal brain ischemia in the rat is involved in neuroprotection afforded by 17p-estradiol Diana Amantea1 Paola Spagnuolo1 2 Monica Bari2 3 Filomena Fezza2 3 Cinzia Mazzei1 Cristina Tassorelli4 Luigi A. Morrone1 Maria T. Corasaniti3 5 Mauro Maccarrone3 6 and Giacinto Bagetta1 1 Department of Pharmacobiology and University Center for the Study of Adaptive Disorder and Headache UCADH Section of Neuropharmacology of Normaland PathologicalNeuronalPlasticity University of Calabria Rende CS Italy 2 Department of ExperimentalMedicine and BiochemicalSciences University of Rome Tor Vergata Rome Italy 3 IRCCS NeurologicalInstitute C. Mondino Foundation Mondino-Tor Vergata Center for ExperimentalNeuropharmacology Laboratory of Neurochemistry Rome Italy 4 Laboratory of Pathophysiology of Integrative Autonomic Systems IRCCS NeurologicalInstitute C. Mondino Foundation and University Centre for the Study of Adaptive Disorder and Headache UCADH Pavia Italy 5 Department of PharmacobiologicalSciences University Magna Graecia of Catanzaro Italy 6 Department of BiomedicalSciences University of Teramo Italy Keywords endocannabinoids estrogen middle cerebral artery occlusion stroke Correspondence G. Bagetta Department of Pharmacobiology University of Calabria via P. Bucci Ed. Polifunzionale 87036 Rende CS Italy Fax 39 0984 493462 Tel 39 0984 493462 E-mail g.bagetta@unical.it These authors contributed equally to this work Received 28 March 2007 revised 18 June 2007 accepted 3 July 2007 doi 10.1111 j.1742-4658.2007.05975.x Endogenous levels of the endocannabinoid anandamide and the activities of the synthesizing and hydrolyzing enzymes i.e. N-acylphosphatidyletha-nolamine-hydrolyzing phospholipase D and fatty acid amide hydrolase respectively were determined in the cortex and the striatum of rats subjected to transient middle cerebral artery occlusion. Anandamide content was markedly increased 3-fold over controls P 0.01 in the .