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Báo cáo y học: "Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, Apis mellifera"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, Apis mellifera | Virology Journal BioMed Central Open Access Methodology Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee Apis mellifera Humberto F Boncristiani Jr1 Gennaro Di Prisco2 Jeffery S Pettis1 Michele Hamilton and Yan Ping Chen 1 Address 1USDA-ARS Bee Research Laboratory Beltsville MD 20705 USA and 2Dipartimento di Entomologia e Zoologia Agraria Filippo Silvestri1 - Via Università n. 100 80055 Portici Napoli Italy Email Humberto F Boncristiani - humberto.boncristianai@ars.usda.gov Gennaro Di Prisco - gennaro.diprisco@unina.it Jeffery S Pettis - jeff.pettis@ars.usda.gov Michele Hamilton - Michele.hamilton@ars.usda.gov Yan Ping Chen - judy.chen@ars.usda.gov Corresponding author Published II December 2009 Received 24 August 2009 Accepted 1 1 December 2009 Virology Journal 2009 6 22 1 doi 10.1186 1743-422X-6-22 1 This article is available from http www.virologyj.cOm content 6 1 221 2009 Boncristiani et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background For years the understanding of the pathogenetic mechanisms that underlie honey bee viral diseases has been severely hindered because of the lack of a cell culture system for virus propagation. As a result it is very imperative to develop new methods that would permit the in vitro pathogenesis study of honey bee viruses. The identification of virus replication is an important step towards the understanding of the pathogenesis process of viruses in their respective hosts. In the present study we developed a strand-specific RT-PCR-based method for analysis of Deformed Wing Virus DWV replication in honey bees and in honey bee parasitic mites Varroa Destructor. Results The results shows that the method developed in .