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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway"
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway | BMC Plant Biology BioMed Central Open Access Research article The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway Cinzia Comino1 Alain Hehn2 Andrea Moglia1 Barbara Menin1 Frédéric Bourgaud2 Sergio Lanteri1 and Ezio Portis 1 Address 1DiVaPRA Plant Genetics and Breeding University of Torino 10095 Grugliasco Torino Italy and 2UMR 1121 Nancy Université INPL -INRA Agronomie Environnement Nancy-Colmar 2 avenue de la Forêt de Haye 54505 Vandoeuvre-lès-Nancy France Email Cinzia Comino - cinzia.comino@unito.it Alain Hehn - alain.hehn@ensaia.inpl-nancy.fr Andrea Moglia - andrea.moglia@unito.it Barbara Menin - barbara.menin@unito.it Frédéric Bourgaud - frederic.bourgaud@ensaia.inpl-nancy.fr Sergio Lanteri - sergio.lanteri@unito.it Ezio Portis - ezio.portis@unito.it Corresponding author Published 18 March 2009 Received 25 September 2008 BMC Plant Biology 2009 9 30 doi l0.ll86 l47l-2229-9-30 Accepted 18 March 2009 This article is available from http www.biomedcentral.cOm l47l-2229 9 30 2009 Comino et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The leaves of globe artichoke and cultivated cardoon Cynara cardunculus L. have significant pharmaceutical properties which mainly result from their high content of polyphenolic compounds such as monocaffeoylquinic and dicaffeoylquinic acid DCQ and a range of flavonoid compounds. Results Hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase HQT encoding genes have been isolated from both globe artichoke and cultivated cardoon GenBank accessions DQ915589 and DQ915590 respectively using CODEHOP and PCR-RACE. A phylogenetic analysis revealed that their sequences belong to one of the major acyltransferase groups