tailieunhanh - báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress | BMC Plant Biology BioMed Central Research article Open Access Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress Efrén Santos 2 Serge Remyt1 Els Thiry1 Saskia Windelinckx1 Rony Swennen1 and László Sági 1 Address laboratory of Tropical Crop Improvement Division of Crop Biotechnics Katholieke Universiteit Leuven Kasteelpark Arenberg 13 B-3001 Leuven Belgium and 2Current address Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral ESPOL Campus Gustavo Galindo Km. vía Perimetral Apartado 09-01-5863 Guayaquil Ecuador Email Efrén Santos - Serge Saskia Windelinckx - Rony Swennen - László Sági - Corresponding author fEqual contributors Published 24 June 2009 BMC Plant Biology 2009 9 77 doi l47l-2229-9-77 Received 19 January 2009 Accepted 24 June 2009 This article is available from http 147l-2229 9 77 2009 Santos et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Next-generation transgenic plants will require a more precise regulation of transgene expression preferably under the control of native promoters. A genome-wide T-DNA tagging strategy was therefore performed for the identification and characterization of novel banana promoters. Embryogenic cell suspensions of a plantain-type banana were transformed with a promoterless codon-optimized luciferase luc gene and low temperature-responsive luciferase activation was monitored in real time. Results Around l6 000 transgenic cell .