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Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases

InArabidopsis thaliana, the serine⁄threonine protein kinase oxidative signal-inducible 1 (OXI1), mediates oxidative stress signalling. Its activity is required for full activation of the mitogen-activated protein kinases (MAP-Ks), MPK3 and MPK6, in response to oxidative stress. | ijFEBS Journal The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases Celine Forzani1 f Alessandro Carreri1 Ị Sergio de la Fuente van Bentem1 David Lecourieux1 - Fatma Lecourieux1 - and Heribert Hirt1 2 1 Max Perutz Laboratories Vienna Austria 2 URGV Plant Genomics INRA-CNRS-University of Evry France Keywords Arabidopsis thaliana MAPK OXI1 oxidative stress PTI1-4 Correspondence H. Hirt URGV Plant Genomics 2 rue Gaston Cremieux F-91057 France Fax 33 1 60 87 45 10 Tel 33 1 60 87 45 08 E-mail hirt@evry.inra.fr These authors contributed equally to this work Present addresses Cardiff Schoolof Biosciences Biomedical Sciences Building Cardiff UK ỊSICIT 2000 S.p.A. Chiampo Italy Syngenta Seeds Enkhuizen the Netherlands -UMR Ecophysiology and Grape Functional Genomics University of Bordeaux INRA Institut des Sciences de la Vigne et du Vin Villenave d Ornon France Received 24 November 2010 revised 13 January 2011 accepted 26 January 2011 doi 10.1111 j.1742-4658.2011.08033.x In Arabidopsis thaliana the serine threonine protein kinase oxidative signalinducible 1 OXI1 mediates oxidative stress signalling. Its activity is required for full activation of the mitogen-activated protein kinases MAP-Ks MPK3 and MPK6 in response to oxidative stress. In addition the serine threonine protein kinase Pto-interacting 1-2 PTI1-2 has been positioned downstream from OXI1 but whether PTI1-2 signals through MAPK cascades is unclear. Using a yeast two-hybrid screen we show that OXI1 also interacts with PTI1-4. OXI1 and PTI1-4 are stress-responsive genes and are expressed in the same tissues. Therefore studies were undertaken to determine whether PTI1-4 is positioned in the OXI1 MAPK signalling pathway. The interaction between OXI1 and PTI1-4 was confirmed by using in vivo co-immunoprecipitation experiments. OXI1 and PTI1-4 were substrates of MPK3 and MPK6 in vitro. Although no direct interaction was detected