tailieunhanh - báo cáo khoa học: " Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells | BioMed Central BMC Plant Biology Research article Open Access Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells Mari Aidemark Carl-Johan Andersson Allan G Rasmusson and Susanne Widell Address Department of Cell and Organism Biology Lund University Sõlvegatan 35 SE-223 62 Lund Sweden Email Mari Aidemark - Carl-Johan Andersson - Allan G Rasmusson - Susanne Widell - Corresponding author Published 12 March 2009 Received 3 October 2008 BMC Plant Biology 2009 9 27 doi l47l-2229-9-27 Accepted 12 March 2009 This article is available from http l47l-2229 9 27 2009 Aidemark et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The cell wall component callose is mainly synthesized at certain developmental stages and after wounding or pathogen attack. Callose synthases are membrane-bound enzymes that have been relatively well characterized in vitro using isolated membrane fractions or purified enzyme. However little is known about their functional properties in situ under conditions when the cell wall is intact. To allow in situ investigations of the regulation of callose synthesis cell suspensions of Arabidopsis thaliana Col-0 and tobacco BY-2 were permeabilized with the channel-forming peptide alamethicin. Results Nucleic acid-binding dyes and marker enzymes demonstrated alamethicin permeabilization of plasma membrane mitochondria and plastids also allowing callose synthase measurements. In the presence of alamethicin Ca2 addition was required for callose synthase activity and the activity was further stimulated by Mg2 .