tailieunhanh - báo cáo khoa học: " In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots | BMC Plant Biology BioMed Central Open Access In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots Stefano Gattolin Mathias Sorieul Paul R Hunter Roman H Khonsari and Lorenzo Frigerio Address Department of Biological Sciences University of Warwick Coventry CV4 7AL UK Email Stefano Gattolin - Mathias Sorieul - Paul R Hunter - paul_hunter@ Roman H Khonsari - Lorenzo Frigerio - Corresponding author Published 18 November 2009 Received 29 June 2009 BMC Plant Biology 2009 9 133 doi l47l-2229-9-l33 Accepted 18 November 2009 This article is available from http l47l-2229 9 l33 2009 Gattolin et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Tonoplast intrinsic proteins TIPs are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For several isoforms the tissue and cell specific pattern of expression are not known. Results We generated fluorescent protein fusions to the genomic sequences of all members of the Arabidopsis TIP family whose expression is predicted to occur in root tissues TIPl l and l 2 TIP2 l 2 2 and 2 3 TIP4 l and expressed these fusions both individually and in selected pairwise combinations in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP distribution varied between different cell layers within the root axis with extensive co-expression of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose expression overlapped appeared to localise to the tonoplast of the central vacuole vacuolar bulbs and .