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In vitro regeneration of capsicum (Capsicum annuum L.) from cotyledon explants

Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium. The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively). | Int.J.Curr.Microbiol.App.Sci 2017 6 5 225-237 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 5 2017 pp. 225-237 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2017.605.026 In Vitro Regeneration of Capsicum Capsicum annuum L. from Cotyledon Explants Vivek Hegde1 3 P.S. Partap1 and R.C. Yadav2 1 Department of Vegetable Science CCS Haryana Agricultural University Hisar-125 004 Haryana India 2 Department of Molecular Biology and Biotechnology CCS Haryana Agricultural University Hisar-125 004 Haryana India 3 Division of Crop Improvement ICAR- Central Tuber Crops Institute Thiruvananthapuram Kerala India Corresponding author ABSTRACT Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by Keywords using cotyledon explants from aseptically raised seedlings of popular Capsicum F 1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper In vitro germination were decontaminated and sown in vitro on half-strength MS medium. The regeneration seed soaked in distilled water along with 2 mg l GA3 for two days prior to sowing had Cotyledon more pronounced effect on both capsicum hybrids recording maximum germination 90.45 Capsicum and 84.59 respectively in minimum number of days 9.67 days and 10.33 days Tissue culture. respectively . Regeneration potential of cotyledonary explants touching the medium both Article Info abaxial and adaxial sides was examined. Tissue culture responses varied with the genotypes side of explants touching the medium and combinations of growth regulators Accepted used. Regeneration per cent 96.30 number of shoots per explants 4.56 and per cent 04 April 2017 shoot elongation 82.10 was maximum in hybrid Indra from cotyledon explant having Available Online abaxial side in contact with growing .