tailieunhanh - Multiple shoot regeneration of plumular apices of chickpea

Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 33-39 © TÜBİTAK doi: Multiple shoot regeneration of plumular apices of chickpea 1, 2 2 2 Muhammad AASIM *, Sibel DAY , Fereshteh REZAEI , Mortaza HAJYZADEH Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Yunus Emre Campus, 70200 Karaman, Turkey 2 Department of Field Crops, Faculty of Agriculture, Ankara University, 06110, Dışkapı, Ankara, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies. It is a highly recalcitrant plant and there is an urgent need to develop a regeneration protocol that can ensure easy multiple and qualitative superior shoots that could be rooted and yield fertile plants. Unconditioned plumular apices and plumular apices preconditioned with 10 mg L–1 benzylaminopurine (BA) for 10 days were cultured on Murashige and Skoog (MS) medium supplemented with – mg L–1 BA with or without mg L–1 naphthalene acetic acid (NAA). Comparing the 2 culture conditions, the preconditioned explants had 2-fold to 5-fold more regeneration and shoots per explant compared to the unconditioned plumular apices. The presence of NAA in the culture medium positively increased the number of shoots per preconditioned explant at the lower concentrations of BA. In contrast, NAA inhibited the number of shoots per explants and the mean shoot length of the unconditioned explants at all concentrations of BA. An increased sucrose concentration of 45 and 60 g L–1 (R1) with mg L–1 indole-3 butyric acid resulted in 50% rooting with multiple and earlier hardening of shoots. .