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Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones

The present investigation was conducted to study the effects of auxin and cytokinin on callus induction from leaf blade, nodal, and runner segments of 3 strawberry clones. Among the 32 media combinations tested, 4.0 mg/L NAA + 1.5 mg/L IBA yielded the highest percentage of callus in all types of explants. | Turk J Biol 34 (2010) 75-80 © TÜBİTAK doi:10.3906/biy-0708-7 Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones Manosh Kumar BISWAS1, Uthpal Krishna ROY2, Rafiul ISLAM2, Monzur HOSSAIN2 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, CHINA 2Department of Botany, University of Rajshahi, BANGLADESH Received: 15.08.2007 Abstract: The present investigation was conducted to study the effects of auxin and cytokinin on callus induction from leaf blade, nodal, and runner segments of 3 strawberry clones. Among the 32 media combinations tested, 4.0 mg/L NAA + 1.5 mg/L IBA yielded the highest percentage of callus in all types of explants. The rate of adventitious shoot regeneration from callus was also influenced by explant type and concentration of BA in MS medium. The highest shoot regeneration rate was observed in leaf derived callus of clone Rabi-01 on media having 3 mg/L BA. Adventitious shoots were rooted, acclimatized, and subsequently transplanted to the field and observed until fruit production. Key words: Auxin, cytokinin, callus, shoot regeneration, strawberry Üç değişik çilek (Fragaria sp.) klonunda yaprak sapı, boğum ve stolon parçalarından kallus kültürü Özet: Bu araştırmada, üç değişik çilek klonuna ait yaprak sapı, boğum ve stolon parçalarından kallus elde edilmesi üzerine oksin ve sitokininin etkileri ortaya konulmuştur. Denenen otuz iki besin ortamı kombinasyonu arasında 4,0 mg/L NAA + 1,5 mg/L IBA, tüm eksplant tiplerinde en yüksek kallus oluşum oranını vermiştir. Kallustan adventif sürgün oluşum oranı, MS besin ortamındaki IBA konsantrasyonundan ve eksplant tipinden etkilenmiştir. En yüksek sürgün oluşum oranı Rabi-01 klonuna ait yapraktan elde edilen kalluslarda ve 3 mg/L BA içeren ortamlar üzerinde elde edilmiştir. Adventif sürgünler köklendirilmiş, dış koşullara alıştırılmış ve daha sonra da araziye aktarılarak meyve üretimine .