tailieunhanh - Callus induction of kappaphycus alvarezii collected from Khanh Hoa province by tissue culture

Tissue culture techniques were applied for inducing callus of the red alga Kappaphycus alvarezii. The objective of this study was to obtain the optimal medium for callus induction from thallus explants of Kappaphycus alvarezii. Seaweed was collected from the Van Phong Bay (Khanh Hoa Province) and then was acclimatized in greenhouse and in semi-sterile culture in the laboratory. | Journal of Fisheries science and Technology Special issue - 2015 CALLUS INDUCTION OF KAPPAPHYCUS ALVAREZII COLLECTED FROM KHANH HOA PROVINCE BY TISSUE CULTURE Khuc Thi An1, Van Hong Cam1 ABSTRACT Tissue culture techniques were applied for inducing callus of the red alga Kappaphycus alvarezii. The objective of this study was to obtain the optimal medium for callus induction from thallus explants of Kappaphycus alvarezii. Seaweed was collected from the Van Phong Bay (Khanh Hoa Province) and then was acclimatized in greenhouse and in semi-sterile culture in the laboratory. Sterilized explants were cultured on PES, ESS and CW media solidified with Bacto Agar. The result indicated that the optimal medium for callus induction was PES solidified medium. In the PES medium, combinations of plant growth regulators . BAP, IAA and NAA were added. Types of callus formed were: filamentous callus and compact callus. Time and percentage of callus formation were different depending on the concentration, type of phytohormone, and type of callus as well. In particular, BAP 1 mg/l + IAA 5 mg/l induced fillamentous callus with but no compact callus induced after 22 days while BAP 0 mg/l + NAA mg/l induced fillamentous callus and 10% of compact callus after 19 days. Our results can be the premise for next researches on multiplying selected strains and increasing seed stocks production of Kappaphycus alvarezii. Keywords: Kappaphycus alvarezii, tissue culture, callus, seaweed, plant growth hormones I. INTRODUCTION Kappaphycus alvarezii is an important source of the industrial gel carrageenan. It is one of the important commercial species cultivated in Southeast Asia. In Vietnam, Kappaphycus alvarezii is cultured in the Central of Vietnam. Many places in Khanh Hoa province grow this commercial species. However, dwindling resources of seedstocks, loss of genetic variability, and fastspreading diseases has significant negative impacts on the carrageenophyte seaweed