tailieunhanh - Efficient callus induction through anther culture in cultivars of Brassica Campestris var. Brown Sarson

In the present study, anther of three varieties and their cross combinations of Brassica campestris, namely HPBS-1, KBS-3, HPKM-04-1, HPBS-1 x HPKM-04-1 and KBS-3 x HPKM-04-1 were cultured in vitro to observe their callus induction frequency. The effect of two basal media . MS and N6, two different sucrose concentrations . 3% and 4%, three combinations of growth hormones viz. HM1, HM2, HM3 and their callus induction frequency were analyzed by CPCS software. Out of all factors and their interactions, the genotype HPBS-1 performed better in N6 medium with HM2 [ mg/l 2, 4-D + mg/l NAA] and 3 per cent sucrose for callus induction frequency. This media and hormonal combination can be successfully utilized for induction of haploids and double haploids in future. | Efficient callus induction through anther culture in cultivars of Brassica Campestris var. Brown Sarson

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