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Dipeptidyl peptidase IV production by solid state fermentation using alternative fungal sources
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The present work was carried out for the production of dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) using Aspergillus Penicillium, and Rhizopus strains under solid state fermentation conditions. | Turk J Biol 36 (2012) 665-671 © TÜBİTAK doi:10.3906/biy-1201-26 Dipeptidyl peptidase IV production by solid state fermentation using alternative fungal sources Gaye ÖNGEN, Sayıt SARGIN, Özlem ÜSTÜN, Ceren KUTLU, Mesut YÜCEL Department of Bioengineering, Faculty of Engineering, Ege University, 35100 İzmir - TURKEY Received: 23.01.2012 ● Accepted: 13.06.2012 Abstract: The present work was carried out for the production of dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) using Aspergillus, Penicillium, and Rhizopus strains under solid state fermentation conditions. Response surface methodology was applied for the optimization of the selected operational variables (corn flour, initial moisture content, and cultivation time) for DPP IV activity as the response. The optimal parameters of DPP IV activity for the independent variables, namely the amount of corn flour (% w/w), initial moisture content (% w/w), and cultivation time (days), were evaluated to be 2.44%, 60.85%, and 4.69 days, respectively, using Aspergillus awamori T116. The response for these results was also shown to be in very close agreement with the experimental data. Key words: Dipeptidyl peptidase IV, Aspergillus, Penicillium, Rhizopus, solid state fermentation Introduction Studies on the dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) (CD26) enzyme have focused especially on human health. Besides being a useful marker for early diagnosis of diabetic nephropathy and tumor burden, DPP IV has roles in many biological processes including pancreatic cancer and rheumatoid arthritis (1-9). In addition to mammalian cells, DPP IV was isolated from insect and microbial cells (10). Mentlein mentioned the interaction of the DPP IV (CD26) enzyme with mammalian regulatory peptides in detail, and also mentioned that DPP IV had an important role not only in the regulation of the endocrine system, immune system, and gastrointestinal system, but also in the regulation of the nervous system (11). In humans, DPP IV was .