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Introduction to Modern Liquid Chromatography, Third Edition part 83

Introduction to Modern Liquid Chromatography, Third Edition part 83. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 776 SAMPLE PREPARATION micropipette tip 96-well SPE plate coated fibers a stir-bar sorbent extraction The micropm Cto tpp MPT format Fig. 16.2c permits the handling of submicroliter amounts of sample such as biological fluids. Solid-phase extraction SPE has been oerformeelwnh various paekrngs that are placed in the pipette up nr embedded in or coated on the internal walls of the tip. With coatings on or embedded in the internal surface orthetip liquidramples can be drawn up and expelled without undo pressure drop orplugging.Sample isdrawo inro phe tip where p interactswith the SPE packing.Nexithe tip may ite rinsedtoeaSancocleptiah dienes elutedwirh a strong solveanJO a npipqoe iareHiudt k i etl vei nadt rtdtP PeiaTe inctucPng reversed-phese-eipn exchangeiihpdtophoPécinttaectiwn- hydroptolicmteahcllon- immobilizedcmetat afhmtyeJ epPaffiastptchlpmha0aeophy.MPTt dnemamliTveei used for pueiPcation cnnpenleaeéea andrelecTve -solation e.p. efdmpy meiai chelation oP paoteínsand ppptide annr eTn uhPattPTW Poa MAPDSpud Poe other advanciU StP qrus PP P1 . One pasUv mpinadponPagaehPmicropipette tips is ahatpdeyesaOausedwiphmicIapipssssreOTip liqutd-havdling sntpmatian. The m epote SPE plate isaaothaudiskPcsmotiahc den-ts weisuárd lor autamatiananW tk PlTpmiTsam u pi alip-uC pumhcpol pmsilsamples.In thiu Parmat 96tlowiphropwhedE irrUe ppO.npa UmL velumeuornam smp-1 masses aP packing opuand a00 mg paniaipph by small Prlpp w opmxlUddiru fitph iWaai disks. The plaietr andoaousPoa8 xt2aaedp pi inmiaeurioedSPE 0 18. The plates caa boh ai dkS byrobotici a rmmt p ti twcamalelelp corcmattthe SPE proces1lDedicaieh SGtopnpoiipn-ationttdmme tap uptameticaliaavaherate the elutiotisnlvent Prmd caliectsog olattp. Thm yvapmradyd ssm letd í ai tm te reconstituetdwlpha seliMe injeytiontolveatand ipseier direcilpPpampne96iWdll plate. Coatsd pOrcp are ppedfersplid-pppte micepex1uarcipu ISaMEp. Io íps desiga a fiised-tiUca fibep ia cpettW witt applymprlc sraduhald phap ruah es a .