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Introduction to Modern Liquid Chromatography, Third Edition part 25

Introduction to Modern Liquid Chromatography, Third Edition part 25. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 196 DETECTION Figure 4.37 shows an example of the popular post-column derivatization of amino acids with ninhydrin followed by UV detection at 405 nm. In this case 45 amino acids and related substances are separated on an ion exchange column followed by reaction with ninhydrin at 130 C in a 500-itL reactor residence time 45 sec . Sample derivatization and reaction detectors have gradually lost popularity as other HPLC detectorshavebeen introduced andbeenmade moreeffective. REFERENCES 1. G Hesse and H. Wed eds. MzCBofi T-zrodtO PzcoL ho.r oo ChrorneSogrophy M. Woelm Eschwege 1954. 2. V. G. Berezkin ed. Chromatographic Adsorption Analysis Selected Works of M. S. Tswett Ellis Horwood New York 1990. 3. A. Tiselius and D. Claesson Arkiv. Kemi Mineral. GeoL 15B 18 1942 1. 4. A. J. P. Martin aosSO. h.Randall Bzoc0rs77. J. 19 1951 243. 5. C. G. Horvath and C.R.LipsOyiNiZiwre 211 1966 748. 6. J. J. Kirkknd Anal.COem. 4O11908 391. 7. E. Haahti and T. Nikkei. r 7n-7s-ooVDoS. IO .1963 2565. 8. R. P. W.Scott and J.G.La2traniio sS.Chrcmatogr.Sci. ll l77n 65. 9. B. G. Julin H.W. Vandenh.m.and JJ.KkkknhJ.Cdro Oogr. 112 1975 443. 10. J. W. Dotanarnt a.N. Seibe- AyR. Chem. 49 lC77 326. 11. L. R. Snyder andj. P.KiM.ncObrtroductionto Modern Liquid Chromatography 1st ed. Wiley NewYork 1979 p.lh6- 12. J. W. Dotan LCGC L4 eOL6 A78. 13. L. R. Snyder Andji W. to ao HiLhdL.formance Gradien-E Miz o Wil43 NewYor1 2007. 14. J. J. KioOknt W.W.Yau.H.J. Stokioso andC.H. Dilks Ji. J.Chcomatoor Sci. 43 1977 303o 15. J. C. Stomberg in Aifi-iwcoo zo COromilogripLy Vol. 2 J. C. Giddings and R. A. Keller eds. Do2ken NewYork OL66 g. 205. 16. D. Parriott C. Ptactical Guide to HPLC Detection Academic Press San Diego 1993. 17. R. P. W.Seott mHaodbgok of HPLC ChromatograoLiaSciycceSerirs Vol.38 E. i.1ee 2c2. 15. 18. D. T. BachiLSt Pi7O imOi NaW 5O Seo1.09 tkC9 . 19. M. H. OOaric tr.erd Paleht 6 642 237 AnrM 2000 . 20. G. Cameton P. E. Jackson ondM.V.Gosenstein COrncAusS 6O 1993 288. 21. A. G. Frenich Z- R.