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Introduction to Modern Liquid Chromatography, Third Edition part 12

Introduction to Modern Liquid Chromatography, Third Edition part 12. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 66 BASIC CONCEPTS AND THE CONTROL OF SEPARATION present it will be necessary to add a buffer to the mobile phase so as to control its pH pKl values for sample compounds if available can be useful during method development Chapter 7 . The molecular weight of the sample may also affect the choice of separation conditions for initial experiments Sections 9.1.1 13.4.1.4 . Samples that contain enantiomers will require the development of a special method for their separation Chapterl4 . The eepaeationgva s f r asempleneynotbe lindtedto adrqpate resolution and minimum rye sime. Deuendingontle syuiament that is eeailable Serthsioutine applicationet Oiefindmetiindi udient eleiion mgynet bcporsible an socratic method widtieernfoeeberedtnrrni.Teacaanctysis asin toe denrrmmatioyot retm pound impuritirs mnyimpotecdd tionrlrequirements on both sample preparation and the detector.Qnndiitafiveenelpfis cnikeor surnrrninlmom ureeitiun e.g. 1-2 formajor componentsanO 10n2e fortsaesannetituents . Hpweevr often onlyeeme teethe rompieccmponentrweOeeqoieeseodoationiloe rxampie drugs pressneieblcod orueine pi prtsiaiOes inwatee orsorl eirnnlet. 2.54.2 SamptePrelre ment Prior to secae n .of n sera k ta 1 . eemove e ponenrs rharaandamrge tb buenoorm wbOrhr teporanonof dcmprnmds o use of a wide range of separation media. For this reason these procedures can be more diffioultto devclopthan the subsequent HPTC separation. When a pretreatment procedere fora n br comCieaticnoranvlyteeod eamplematrix iravaieabls either as eevrlopediu adr sirnelaboialervor er mpm-ivl inthellteratuee itsp1e or adaptationfortPetcmpleie ofteopreeereaibeocompleer rr-dennlopmsne o a samplc-ptsncetmcc urocedtoeceo Us aeoidel dusapueoavh fieetliOelv lobe applicable lerl U 1 TstoMibma eer te teeaecsion2.O.4.5 . 2.5.4.3 SaleeliodOsChremaSovruoa cModd Reversed-yVcseleetomrtoctaPOy s lbs n e 1 i for HPLd meehad nevefop-ment. However depending on the sample other chromatographic modes may be prcfcrablc.Oftid timcethis docrnt