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Báo cáo khoa học: Calcium modulates endopeptidase 24.15 (EC 3.4.24.15) membrane association, secondary structure and substrate specificity

The metalloendopeptidase 24.15 (EP24.15) is ubiquitously present in the extracellular environment as a secreted protein. Outside the cell, this enzyme degrades several neuropeptides containing from 5 to 17 amino acids (e.g. gonadotropin releasing hormone, bradykinin, opioids and neuro-tensin). The constitutive secretion of EP24.15 from glioma C6 cells was demonstrated to be stimulated linearly by reduced concentrations of extra-cellular calcium. | iFEBS Journal Calcium modulates endopeptidase 24.15 EC 3.4.24.15 membrane association secondary structure and substrate specificity Vitor Oliveira1 Paula A. G. Garrido2 Claudia C. Rodrigues2 Alison Colquhoun2 Leandro M. Castro2 Paulo C. Almeida3 Claudio S. Shida3 Maria A. Juliano4 Luiz Juliano4 Antonio C. M. Camargo5 Stephen Hyslop6 James L. Roberts7 Valerie Grum-Tokars8 Marc J. Glucksman8 and Emer S. Ferro2 1 Laboratorio de Neurociencias Universidade da Cidade de Sao Paulo Brazil 2 Departamento de Biologia Celular e Desenvolvimento Programa de Biologia Celular Instituto de Ciencias Biomedicas Universidade de Sao Paulo Brazil 3 Universidade de Mogi das Cruzes Mogi das Cruzes Brazil 4 Departamento de Biofisica Universidade Federalde Scio Paulo Brazil 5 Centro de Toxinologia Aplicada CAT Instituto Butantan Brazil 6 Departamento de Farmacologia Faculdade de Ciencias Medicas Universidade Estadualde Campinas Campinas Brazil 7 Sam and Ann Barshop Institute for Longevity and Aging Studies University of Texas Health Science Center San Antonio TX USA 8 Midwest Proteome Center Department of Biochemistry Molecular Biology Rosalind Franklin University of Medicine and Science Chicago Medical School USA Keywords calcium membrane binding peptide metabolism protease thimet oligopeptidase Correspondence E. S. Ferro Laboratorio de Comunicacao Celular Avenida Prof. Lineu Prestes1524 Sala 431 Sao Paulo 05508-900 SP Brazil E-mail eferro@usp.br Note V. Oliveira and P.A.G. Garrido contributed equally to this work. Received 9 February 2005 revised 24 March 2005 accepted 31 March 2005 doi 10.1111 j.1742-4658.2005.04692.x The metalloendopeptidase 24.15 EP24.15 is ubiquitously present in the extracellular environment as a secreted protein. Outside the cell this enzyme degrades several neuropeptides containing from 5 to 17 amino acids e.g. gonadotropin releasing hormone bradykinin opioids and neurotensin . The constitutive secretion of EP24.15 from glioma C6 cells was demonstrated to be .