tailieunhanh - Báo cáo y học: "Relating a calcium indicator signal to the unperturbed calcium concentration time-course"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Relating a calcium indicator signal to the unperturbed calcium concentration time-course | Theoretical Biology and Medical Modelling BioMed Central Research Relating a calcium indicator signal to the unperturbed calcium concentration time-course Alexander Borst 1 and Henry DI Abarbanel2 Open Access Address 1Max-Planck-Institute of Neurobiology Martinsried Germany and 2Department of Physics and Marine Physical Laboratory Scripps Institution of Oceanography University of California San Diego USA Email Alexander Borst - borst@ Henry DI Abarbanel - hdia@ Corresponding author Published 6 February 2007 Received 24 October 2006 Theoretical Biology and Medical Modelling 2007 4 7 doi 1742-4682-4-7 Accepted 6 February 2007 This article is available from http content 4 1 7 2007 Borst and Abarbanel licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Optical indicators of cytosolic calcium levels have become important experimental tools in systems and cellular neuroscience. Indicators are known to interfere with intracellular calcium levels by acting as additional buffers and this may strongly alter the time-course of various dynamical variables to be measured. Results By investigating the underlying reaction kinetics we show that in some ranges of kinetic parameters one can explicitly link the time dependent indicator signal to the time-course of the calcium influx and thus to the unperturbed calcium level had there been no indicator in the cell. Background The use of a fluorescent calcium indicator is a familiar technique for detecting dynamical changes in intracellular calcium levels 1-8 . However introduction of the indicator into the cytosol inevitably perturbs the .