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Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds
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Parkia platycephalalectin 2 was purified from Parkia platycephala(Legumi-nosae, Mimosoideae) seeds by affinity chromatography and RP-HPLC. Equilibrium sedimentation and MS showed that Parkia platycephala lectin 2 is a nonglycosylated monomeric protein of molecular mass 29 407 ± 15 Da, which contains six cysteine residues engaged in the for-mation of three intramolecular disulfide bonds. | sFEBS Journal cDNA cloning and 1.75 A crystal structure determination of PPL2 an endochitinase and N-acetylglucosamine-binding hemagglutinin from Parkia platycephala seeds Benildo S. Cavada1 Frederico B. B. Moreno2 Bruno A. M. da Rocha1 3 Walter F. de Azevedo Jr4 Rolando E. R. Castellon1 Georg V. Goersch1 Celso S. Nagano5 Emmanuel P. de Souza1 Kyria S. Nascimento1 Gandhi Radis-Baptista1 Plinio Delatorre3 Yves Leroy6 Marcos H. Toyama7 Vicente P. T. Pinto8 Alexandre H. Sampaio9 Domingo Barettino5 Henri Debray6 Juan J. Calvete5 and Libia Sanz5 1 BioMol-Laboratory Departamento de Bioquimica e Biologia Molecular Universidade Federaldo Ceara Fortaleza Ceara Brazil 2 Departamento de Fisica Universidade EstadualPaulista UNESP Sao Jose do Rio Preto Scio Paulo Brazil 3 Departamento de Ciencias Fisicas e Biologicas Universidade Regionaldo Cariri Fortaleza Ceara Brazil 4 Faculdade de Biociencias Centro de Pesquisas em Biologia Molecular e Funcional PUCRS Porto Alegre Rio Grande do Sul Brazil 5 Instituto de Biomedicina de Valencia CSIC Spain 6 Laboratoire de Chimie Biologique et Unite Mixte de Recherche No. 8576 du CNRS Universite des Sciences et Technologies de Lille France 7 Departamento de Bioquimica Instituto de Biologia Universidade Estadualde Campinas UNICAMP Campinas SP Brazil 8 Faculdade de Medicina Universidade Federaldo Ceara Sobral Brazil 9 Laboratorio de Bioquimica Marinha Departamento de Engenharia de Pesca Universidade Federaldo Ceara Fortaleza Ceara Brazil Keywords endochitinase glycosyl hydrolase family 18 Mimosoideae Parkia platycephala X-ray crystal structure Correspondence B. S. Cavada BioMol-Laboratory Departamento de Bioquimica e Biologia Molecular Universidade Federal do Cearai Fortaleza Ceara Brazil Fax Tel 55 8540089818 E-mail bscavada@ufc.br H. Debray Laboratoire de Chimie Biologique et Unite Mixte de Recherche du CNRS N 8576 Universite des Sciences et Technologies de Lille bailment C-9 59655 Villeneuve D Ascq Cedex Fax 33 320436555 Tel 33 320410108 .